stem cell antibody array  (R&D Systems)

Journal: Human Cell

Article Title: Establishment of Coala: a novel 3D and 2D cancer cell line derived from colorectal cancer liver metastasis

doi: 10.1007/s13577-025-01256-1

Figure Lengend Snippet: Characterization of newly-derived Coala cancer cell line. Cell line was derived from human colorectal cancer liver metastasis ( a ), forms sharply-edged, fast growing colonies in 2D ( b – d ), when embedded into Matrigel matrix, the cell line form spherically-shaped tumorspheres ( e , f ). Image g shows the phenotype of tumorspheres, when Coala cells are cultured in ultra-low attachment plates. Scale bars represent 50 µm ( b – d and k , l ) and 500 µm ( e – g ). Multicolor (M-FISH) analysis of Coala cells showing the abnormal karyotype ( h ). Negative PCR test (detection of multiple mycoplasma species) in Coala cell culture ( i ). Antibody array chip analysis shows the expression of Snail, FoxA2, E-Cadherin and Pdx-1 in Coala cell lysate. Cultured Coala cells show positivity for Cytokeratin 20 (DAPI was used for nuclei staining), scale bar represents 50 µm. Growth curve for Coala cells ( m ) and effect of 5-Fluorouracil treatment ( n ). Image ( o ) showing a tumor formed in athymic mouse 3 weeks after subcutaneous grafting of Coala cells. Images ( p ) and ( r ) show flow cytometry analysis of Lgr5 marker in early passage (P3) of Coala cells. Cells from passage No.21 were used in experiments

Article Snippet: Expression of 19 transcription factors was detected with Stem Cell Antibody Array (RD Systems, UK).

Techniques: Derivative Assay, Cell Culture, Ab Array, Expressing, Staining, Flow Cytometry, Marker

Journal: International journal of cancer

Article Title: Extracellular vesicles secreted by triple-negative breast cancer stem cells trigger premetastatic niche remodeling and metastatic growth in the lungs.

doi: 10.1002/ijc.34447

Figure Lengend Snippet: FIGURE 1 EVsCSC and EVsDCC isolation and characterization. (A) Representative images of CSCs and DCCs analyzed for tdTomato fluorescence, scale bar = 25 μm. (B) Gene expression of stemness reporters ALDH1A1, Nanog and Oct-4 detected by qPCR for MDA-MB-231 subpopulations. CSC refers to tdTomato + cells cultured in stem cell maintenance media while DCC indicates cells cultured with a minimal presence of CSC (1%-2%) to avoid de-differentiation. CNRQ stands for Calibrated Normalized Relative Quantity with respect to GAPDH and Actin housekeeping genes. (C) 2D laminin invasion assay comparing CSC and DCC cells. (D) CryoTEM imaging of isolated EVsCSC and EVsDCC. (E) Stochastic Optical Resolution Microscopy (STORM) imaging of isolated EVs previously labeled with DiD. (F) Size distribution by nanoparticle tracking analysis (NTA) of EVsCSC and EVsDCC. (G) EVs typical markers, CD81, TSG101 and ALIX and cell lysate control, β-tubulin identified by Western blot. Twenty micrograms of total protein was loaded per lane and cell extracts (CE) were included as controls. (H) ALDH1A1 and CD44 protein cargo in EVCSC and EVsDCC examined by Western blot. (I) Protein cargo of EVCSC and EVsDCC assayed with a Human Pluripotent Stem Cell Array, showing higher content of OCT4, SOX2 and E- Cadherin in EVCSC. In H and I, quantification of blots are shown as the fold change in the band/dot intensity of EVsCSC/EVsDCC. Differences are significant for ALDH1A1, CD44, SOX2 and E-Cadherin (*P < .05)

Article Snippet: Human pluripotent stem cell antibody array (R&D Systems, ARY010) was performed in accordance with the manufacturer's instructions.

Techniques: Isolation, Fluorescence, Gene Expression, Cell Culture, Invasion Assay, Imaging, Microscopy, Labeling, Control, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice

doi: 10.3390/ijms24054490

Figure Lengend Snippet: PACAP polarizes MSC towards an anti-inflammatory (MSC2) phenotype. Conditioned medium from naïve and PACAP-treated MSC was analyzed for chemokines and cytokines secretion, utilizing Proteome Profiler Mouse Cytokine Array (R&D Systems). The following chemokine and anti-inflammatory cytokines IL-2, IL-3, IL-4, IL-27, IP10, IL-1ra, RANTES, SDF-1, CCL2(JE), CCL1 (i-309), G-CSF, BLC were upregulated, while pro-inflammatory cytokines were downregulated (IL-6, IL-1a, IFN-ɣ and soluble ICAM-1). Analysis was performed on pooled samples (n = 3) for each culture.

Article Snippet: Cytokine expression was detected in 100 μL of serum or pooled conditioned media using the Proteome Profiler Mouse Cytokine Array Kit (R&D Systems, Cat no. SC018), according to the manufacturer’s protocol.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Polarized Anti-Inflammatory Mesenchymal Stem Cells Increase Hippocampal Neurogenesis and Improve Cognitive Function in Aged Mice

doi: 10.3390/ijms24054490

Figure Lengend Snippet: Systemic administration of polarized MSC to aged mice normalizes systemic chemokine levels. Intravenous injection of polarized anti-inflammatory MSC (pMSC, N = 3–4) reduces the levels of plasma chemokines that are associated with aging and inflammation to levels of young (3 months old) mice (N = 7–8) compared with vehicle-treated aged control mice (N = 3), as detected by Proteome Profiler Mouse Cytokine Array (R&D Systems). Bars in the graph present mean ± SE. * p < 0.05, ** p < 0.01. One-way ANOVA. ns = non-significant.

Article Snippet: Cytokine expression was detected in 100 μL of serum or pooled conditioned media using the Proteome Profiler Mouse Cytokine Array Kit (R&D Systems, Cat no. SC018), according to the manufacturer’s protocol.

Techniques: Injection, Clinical Proteomics, Control